Information ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.Usage Physical State: Freeze-dried solid Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity:Based on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against rabbit anti-goat whole serum is the same as that against rabbit anti-goat IgG, Fc fragment specific. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Conjugate DyLight? 405
Amax: 400Emax: 421nmDyLight 405-conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use is limited to microscopes or flow cytometers equipped with a 405 nm laser and a 420 nm emission filter. Under these conditions, it is possible to perform effective 4-color imaging with good color separation, good photostability, and high sensitivity. The combination of DyLight 405, Alexa Fluor? 488, Rhodamine Red-X, and Alexa Fluor? 647 provides for maximum color separation. Another 4-color dye combination, which may be equally effective but has slightly less color separation, is DyLight 405, Alexa Fluor? 488, Cy3, and Alexa Fluor? 647. DyLight 405 conjugates are an excellent choice for blue-fluorescing secondary antibodies in multi-color labeling protocols.
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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
DyLight™ is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
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