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Millipore/04-1088 | Anti-Caspase-3 (Pro) Antibody, clone E83-103, rabbit monoclonal/04-1088/100 µL
  • Millipore/04-1088 | Anti-Caspase-3 (Pro) Antibody, clone E83-103, rabbit monoclonal/04-1088/100 µL

Millipore/04-1088 | Anti-Caspase-3 (Pro) Antibody, clone E83-103, rabbit monoclonal/04-1088/100 µL

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貨號: 04-1088
品牌: Millipore
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    • Description
      CatalogueNumber04-1088
      DescriptionAnti-Caspase-3(Pro)Antibody,cloneE83-103,rabbitmonoclonal
      AlternateNames
      • CysteineproteaseCPP32
      • PARPcleavageprotease
      • SREBPcleavageactivity1
      • Yamaprotein
      BackgroundInformationCaspase-3(CPP32,Apopain)isthemostextensivelystudiedapoptoticprotein.Caspase-3issynthesizedasaninactiveproenzyme(32kDa)thatisprocessedincellsundergoingapoptosisbyself-proteolysisand/orcleavagebyanotherupstreamprotease.Theprocessedformofcaspase-3consistsoflarge(17kDa)andsmall(12kDa)subunitswhichassociatetoformanactiveenzyme.Theactivecaspase-3proteolyticallycleavesandactivatesothercaspases,aswellasrelevanttargetsinthecellssuchasPARPandDFF.
      ProductInformation
      FormatUnpurified
      Control
      • Jurkatlysates
      PresentationRabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.Anti-Caspase-3(Pro)Antibody,cloneE83-103,RabbitisanantibodyagainstCaspase-3(Pro)foruseinWB,IP,IH(P),IC,FC.
      KeyApplications
      • WesternBlotting
      • Immunoprecipitation
      • Immunohistochemistry(Paraffin)
      • Immunocytochemistry
      • FlowCytometry
      ApplicationNotesImmunohistochemistryAnalysis:1:250dilutionfromapreviouslotdetectedpro-caspase-3inhumancolonadenocarcinomatissue.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoresiduesfollowingSer29ofhumancaspase-3(N-terminusofp17subunit).
      EpitopeN-terminus
      CloneE83-103
      HostRabbit
      SpecificityThisantibodyonlydetectspro-form(35kD)ofcaspase-3,anddoesnotrecognizeanycleavedcaspases.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein.[providedbyRefSeq].
      GeneSymbol
      • Apopain
      • CASP-3
      • CPP-32
      • CPP32
      • CPP32B
      • EC3.4.22.56
      • OTTHUMP00000165054
      • SCA-1
      • Yama
      • apopain
      • procaspase3
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp-|-Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin.

      CATALYTICACTIVITY:StrictrequirementforanAspresidueatpositionsP1andP4.IthasapreferredcleavagesequenceofAsp-Xaa-Xaa-Asp-|-withahydrophobicamino-acidresidueatP2andahydrophilicamino-acidresidueatP3,althoughValorAlaarealsoacceptedatthisposition.

      ENZYMEREGULATION:Inhibitedbyisatinsulfonamides.

      SUBUNITSTRUCTURE:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit.

      SubcellularlocationCytoplasm.

      TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem.

      PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa.

      S-nitrosylatedonitscatalyticsitecysteineinunstimulatedhumancelllinesanddenitrosylateduponactivationoftheFasapoptoticpathway,associatedwithanincreaseinintracellularcaspaseactivity.Fasthereforeactivatescaspase-3notonlybyinducingthecleavageofthecaspasezymogentoitsactivesubunits,butalsobystimulatingthedenitrosylationofitsactivesitethiol.

      SEQUENCESIMILARITY:BelongstothepeptidaseC14Afamily.

      MolecularWeight~35kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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