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Millipore/04-802 | Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal/04-802/100 µL
  • Millipore/04-802 | Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal/04-802/100 µL

Millipore/04-802 | Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal/04-802/100 µL

價(jià)格: ￥3576.00 市場(chǎng)價(jià): 5960.00
貨號(hào): 04-802
品牌: Millipore
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    • Description
      CatalogueNumber04-802
      Replaces05-802
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-phospho-Akt1/PKBα(Thr308)Antibody,cloneNL50,rabbitmonoclonal
      AlternateNames
      • ProteinkinaseB
      • RAC-alphaserine/threonine-proteinkinase
      • murinethymomaviral(v-akt)oncogenehomolog-1
      • racproteinkinasealpha
      • v-aktmurinethymomaviraloncogenehomolog1
      BackgroundInformationAktisthemajorknowneffectorofthePI3Kinasepathway.GenerationofPIP3resultsintheactivationofPDK1,whichphosphorylatesAktonThr308,andanotherkinasecomplex,thoughttobethemTORC2complex(orpossIBLyanintermediatekinaselinkingthetwo)thatphosphorylatesAktonSer473.ThesephosphorylationsadditivelyactivateAktSer/Thrkinaseactivity,andtheuseofphosphorylationstate-specificantibodiesdirectedagainsteitherofthesesitescanimplyAktactivation.ActivationofAktcanbemeasureddirectlybyimmunoprecipitationfollowedbyphosphorylationofaknownsubstratewithrADIolabeledATP.Akthasbeenshowntophosphorylateover70substratesincludingTSC1&2inthemTORpathway,BadonSer136,GSK3,whichisinactivatedbythisphosphorylation,theFoxOfamilyoftranscriptionfactors,PRAS40,AS160.
      ProductInformation
      FormatCultureSupernatant
      Control
      • PDGFtreated3T3/NIHcells
      PresentationCulturedsupernatantcontaining0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationThisAnti-Akt1Antibody,cloneNL50isvalidatedforuseinWBforthedetectionofphospho-Akt1/PKBα(Thr308).
      KeyApplications
      • WesternBlotting
      ApplicationNotesImmunoblotAnalysis:A1:500-1:2,000dilutionofthislotdetectedphospho-Akt1/PKBαinRIPAlysatesfrom3T3/NIHcellsstimulatedwith100ng/mlPDGFfor20minutes(FigureA).
      Beadlyte?SpecificityAssay:1:1,000-1:10,000dilutionsofthislotwereincubatedwithphosphorylatedandunphosphorylatedrecombinantAktproteinsconjugatedtoLuminexmicrospheres.Cross-reactivitytoactiveAkt2wasdetected(FigureB).
      BIOLOGicalInformation
      ImmunogenKLH-conjugated,syntheticpeptidecontainingthesequence..MK[pT]FC..inwhichpTcorrespondstophospho-threonineatresidue308ofmouseAkt.TheAkt1sequenceisidenticaltohuman,rat,bovineandchickenAkt1.HumanandmouseAkt2shares11/12
      aminoacidsandratAkt3shares10/12aminoacidswiththepeptideimmunogensequence.
      EpitopepThr308
      CloneNL50
      HostRabbit
      SpecificityRecognizesphosphorylatedAkt1/PKBα,Mr60kDa.Cross-reactivityobservedwithAkt2,butnotwithAkt3.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      • Bovine
      • Chicken
      • Rat
      SpeciesReactivityNoteHumanandmouse.Predictedtocross-reactwithrat,bovineandchickenbasedonsequencehomology.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene.
      GeneSymbol
      • AKT1
      • RAC
      • PRKBA
      • RAC-ALPHA
      • PKB
      • MGC99656
      • RAC-PK-alpha
      • C-AKT
      • AKT
      Modifications
      • Phosphorylation
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P31749#Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI(3)K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesis,partlybyplayingaroleinbothinsulin-inducedphosphorylationof4E-BP1andininsulin-inducedactivationofp70S6kinase.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase.
      SIZE:480aminoacids;55686Da
      SUBUNIT:InteractswithCENTG1isoform2(PIKE-A)inthepresenceofguaninenucleotides.TheC-terminusinteractswithCCDC88A/GRDN.InteractswithAKTIP.
      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Note=Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1).
      TISSUESPECIFICITY:Inallhumancelltypessofaranalyzed.
      DOMAIN:SwissProt:P31749BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI(3)K)resultsinitstargetingtotheplasmamembrane.
      PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.Ser-473phosphorylationbytheRictor-mTorcomplexfavorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithCENTG1isoform2(PIKE-A).
      SIMILARITY:Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.RACsubfamily.&Contains1AGC-kinaseC-terminaldomain.&Contains1PHdomain.&Contains1proteinkinasedomain.
      MolecularWeight~60kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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