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Millipore/05-457 | Anti-Histone H1 Antibody, clone AE-4/05-457/200 µg
  • Millipore/05-457 | Anti-Histone H1 Antibody, clone AE-4/05-457/200 µg

Millipore/05-457 | Anti-Histone H1 Antibody, clone AE-4/05-457/200 µg

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貨號: 05-457
品牌: Millipore
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    • Description
      CatalogueNumber05-457
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-HistoneH1Antibody,cloneAE-4
      AlternateNames
      • H1histonefamily,member4
      • HistoneH1b
      • histone1
      • H1e
      • histonecluster1
      • H1e
      BackgroundInformationHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.
      ProductInformation
      FormatPurified
      Control
      • NuclearextractsfromNIH3T3orHeLacells.
      PresentationPurifiedmousemonoclonalIgG2ainbuffercontainingPBS,pH7.2,with0.1%sodiumazide.Frozensolution.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.

      HandlingRecommendations:
      Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationUseAnti-HistoneH1Antibody,cloneAE-4(mousemonoclonalantibody)validatedinELISA,ICC,WBtodetectHistoneH1alsoknownasH1histonefamilymember4,HistoneH1b,histone1H1e,histonecluster1H1e.
      KeyApplications
      • ELISA
      • Immunocytochemistry
      • WesternBlotting
      ApplicationNotesImmunocytochemistry:
      5-20μg/mLofapreviouslotshowedpositivenuclearimmunostaininginA431cellsfixedwith4%paraformaldehydeandmethanol.

      ELISA:
      Recommended.
      BIOLOGicalInformation
      ImmunogenNucleifrommyeloidleukemiabiopsycells.CloneAE-4.
      CloneAE-4
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityThisantibodyrecognizeshistoneH1,Mr32-33kDa.DoubletdetectedduetohyperphosphorylationofhistoneH1subpopulation.Cross-reactswithotherhistoneisoformsinthefollowingorderofsignalstrength:H3,H2B>H4>H2A,asdeterminedwithrecombinantprotein.
      IsotypeIgG2a
      SpeciesReactivity
      • Human
      • Bovine
      • GreenPlants
      SpeciesReactivityNoteHumanandbovine.Predictedtostainnucleifromotheranimalandplantspecies.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsresponsiblefornucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH1family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6.
      GeneSymbol
      • HIST1H1E
      • H1F4
      • H1.4
      • dJ221C16.5
      • MGC116819
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P10412#HistonesH1arenecessaryforthecondensationofnucleosomechainsintohigherorderstructures.
      SIZE:219aminoacids;21865Da
      SUBCELLULARLOCATION:Nucleus.
      SIMILARITY:SwissProt:P10412##BelongstothehistoneH1/H5family.
      MISCELLANEOUS:Thisvariantaccountsfor60%ofhistoneH1.
      MolecularWeight32-33kDa(HistoneH1);Doublet(hyperphosphorylatedHistoneH1);17kDa(otherhistoneisoforms).
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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