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Millipore/05-480 | Anti-Eck/EphA2 Antibody, clone D7/05-480/200 µg
  • Millipore/05-480 | Anti-Eck/EphA2 Antibody, clone D7/05-480/200 µg

Millipore/05-480 | Anti-Eck/EphA2 Antibody, clone D7/05-480/200 µg

價(jià)格: ￥4812.00 市場價(jià): 8020.00
貨號: 05-480
品牌: Millipore
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    • Description
      CatalogueNumber05-480
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-Eck/EphA2Antibody,cloneD7
      AlternateNames
      • EPHreceptorA2
      • Epithelialcellkinase
      • Tyrosine-proteinkinasereceptorECK
      • ephrinreceptorEphA2
      • epithelialcellreceptorproteintyrosinekinase
      • proteintyrosinekinase
      • receptorproteintyrosinekinaseregulatedbyp53andE2F-1
      BackgroundInformationEPHandEPH-relatedreceptorshavebeenimplicatedinmediatingdevelopmentalevents,particularlyinthenervoussystem.ReceptorsintheEPHsubfamilytypicallyhaveasinglekinasedomainandanextracellularregioncontainingaCys-richdomainand2fibronectintypeIIIrepeats.Theephrinreceptorsaredividedinto2groupsbasedonthesimilarityoftheirextracellulardomainsequencesandtheiraffinitiesforbindingephrin-Aandephrin-Bligands.
      Proteinkinasesareenzymesthattransferaphosphategroupfromaphosphatedonor,generallythegphosphateofATP,ontoanacceptoraminoacidinasubstrateprotein.Bythisbasicmechanism,proteinkinasesmediatemostofthesignaltransductionineukaryoticcells,regulatingcellularmetabolism,transcription,cellcycleprogression,cytoskeletalrearrangementandcellmovement,apoptosis,anddifferentiation.
      ProductInformation
      FormatPurified
      Control
      • A431celllysate,CHOcelllysate,foreskinfibroblasts,3T3/A31cells,L6cells.
        IncludedPositiveAntigenControl:Catalog#12-301,nonstimulatedA431lysate.Add2.5μLof2-mercaptoethanol/100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforminigels.
      PresentationPurifiedmousemonoclonalIgG1inbuffercontaining0.2MTris-glycine,pH7.4,0.15MNaCl,with0.05%sodiumazideand30%glycerol.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.

      HandlingRecommendations:
      Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.NOTE:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerol-containingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationThisAnti-Eck/EphA2Antibody,cloneD7isvalidatedforuseinIC,IP,EA,WBforthedetectionofEck/EphA2.
      KeyApplications
      • Immunocytochemistry
      • Immunoprecipitation
      • EnzymeAssays
      • WesternBlotting
      ApplicationNotesProteinKinaseAssay:
      Apreviouslotofthisantibodyhasbeenreportedtohavebeenusedinanimmunoprecipitationautophosphorylationassay,usingaMn-PIPESreactionbuffer(Romer,L.,1994).

      Immunoprecipitation:
      ApreviouslotofthisantibodyhasbeenreportedtoimmunoprecipitateEckfrom500μgofahumanbreastepithelialcelllinewhichhadbeenlysedinTBScontaining1%TritonX-100.Use1-4μgperreaction.

      Immunocytochemistry:
      ApreviouslotofthisantibodyhasbeenreportedtoimmunostainEckinhuman,mouseandratepithelialcellsfixedwith3.7%formaldehydesolutionandpermeabilizedwith0.5%TritonX-100inTBS.
      BIOLOGicalInformation
      ImmunogenNativeproteinisolatedbypurificationofphosphotyrosine-containingproteins.CloneD7.
      CloneD7
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityThisantibodyrecognizesEpithelialCellKinase(Eck/EphA2),Mr140kDa
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      • Canine
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgenebelongstotheephrinreceptorsubfamilyoftheprotein-tyrosinekinasefamily.EPHandEPH-relatedreceptorshavebeenimplicatedinmediatingdevelopmentalevents,particularlyinthenervoussystem.ReceptorsintheEPHsubfamilytypicallyhaveasinglekinasedomainandanextracellularregioncontainingaCys-richdomainand2fibronectintypeIIIrepeats.Theephrinreceptorsaredividedinto2groupsbasedonthesimilarityoftheirextracellulardomainsequencesandtheiraffinitiesforbindingephrin-Aandephrin-Bligands.Thisgeneencodesaproteinthatbindsephrin-Aligands.
      GeneSymbol
      • EPHA2
      • EphA2
      • ECK
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P29317#Receptorformembersoftheephrin-Afamily.Bindstoephrin-A1,-A3,-A4and-A5.
      SIZE:976aminoacids;108254Da
      SUBUNIT:InteractswithSLA(Bysimilarity).InteractswithINPPL1/SHIP2.
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:Expressedmosthighlyintissuesthatcontainahighproportionofepithelialcells,e.g.,skin,intestine,lung,andovary.
      SIMILARITY:SwissProt:P29317##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.Ephrinreceptorsubfamily.&Contains2fibronectintype-IIIdomains.&Contains1proteinkinasedomain.&Contains1SAM(sterilealphamotif)domain.
      MolecularWeight140kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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