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Millipore/05-710 | Anti-NG2 Antibody, clone 132.38/05-710/200 µg
  • Millipore/05-710 | Anti-NG2 Antibody, clone 132.38/05-710/200 µg

Millipore/05-710 | Anti-NG2 Antibody, clone 132.38/05-710/200 µg

價(jià)格: ￥4680.00 市場(chǎng)價(jià): 7800.00
貨號(hào): 05-710
品牌: Millipore
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    • Description
      CatalogueNumber05-710
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-NG2Antibody,clone132.38
      ProductInformation
      FormatPurified
      Control
      • Braintissue
      PresentationProteinAPurifiedimmunoglobulinin0.1MTris-glycine,pH7.4,0.15MNaClwith0.05%sodiumazideasapreservative.
      StorageandShippingInformation
      StorageConditionsMaintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationAnti-NG2Antibody,clone132.38detectslevelofNG2&hasbeenpublished&validatedforuseinIH&WB.
      KeyApplications
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesCross-reactivityexpectedwithmouse.
      BIOLOGicalInformation
      ImmunogenHEK293cellsexpressingatruncatedintegralmembraneformofNG2consistingofaminoacids1592-2222
      Clone132.38
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizesNG2.
      IsotypeIgG1
      SpeciesReactivity
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryAhumanmelanoma-associatedchondroitinsulfateproteoglycanplaysaroleinstABIlizingcell-substratuminteractionsduringearlyeventsofmelanomacellspreADIngonendothelialbasementmembranes.CSPG4representsanintegralmembranechondroitinsulfateproteoglycanexpressedbyhumanmalignantmelanomacells.
      GeneSymbol
      • CSPG4
      • MCSP
      • HMW-MAA
      • MCSPG
      • MSK16
      • NG2
      • MEL-CSPG
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q6UVK1#Proteoglycanplayingaroleincellproliferationandmigrationwhichstimulatesendothelialcellsmotilityduringmicrovascularmorphogenesis.Mayalsoinhibitneuriteoutgrowthandgrowthconecollapseduringaxonregeneration.Cellsurfacereceptorforcollagenalpha2(VI)whichmayconfercellsabilitytomigrateonthatsubstrate.BindsthroughitsextracellularN-terminusgrowthfactors,extracellularmatrixproteasesmodulatingtheiractivity.MayregulateMPP16-dependentdegradationandinvasionoftypeIcollagenparticipatinginmelanomacellsinvasionproperties.Maymodulatetheplasminogensystembyenhancingplasminogenactivationandinhibitingangiostatin.FunctionsalsoasasignaltransducingproteinbybindingthroughitscytoplasmicC-terminusscaffoldingandsignalingproteins.MaypromoteretractionfiberformationandcellpolarizationthroughRhoGTPaseactivation.Maystimulatealpha-4,beta-1integrin-mediatedadhesionandspreadingbyrecruitingandactivatingasignalingcascadethroughCDC42,ACK1andBCAR1.MayactivateFAKandERK1/ERK2signalingcascades.


      SIZE:2322aminoacids;250497Da

      SUBUNIT:InteractswiththefirstPDZdomainofMPDZ.InteractswithPRKCA.BindsTNC,laminin-1,COL5A1andCOL6A2.InteractswithPLGandangiostatin.BindsFGF2andPDGFA.InteractswithGRIP1,GRIP2andGRIA2.FormsaternarycomplexwithGRIP1andGRIA2(Bysimilarity).InteractswithLGALS3andtheintegrincomposedofITGB1andITGA3.InteractswithITGA4throughitschondroitinsulfateglycosaminoglycan.InteractswithBCAR1,CDC42andACK1.InteractswithMMP16.

      SUBCELLULARLOCATION:Apicalcellmembrane;Single-passtypeImembraneprotein(Bysimilarity).Cellprojection,lamellipodium(Bysimilarity).Note=LocalizedattheapicalplasmamembraneitrelocalizestothelamellipodiaofastrocytomauponphosphorylationbyPRKCA.Localizestotheretractionfibers.Localizestotheplasmamembraneofoligodendrocytes(Bysimilarity).

      TISSUESPECIFICITY:Detectedonlyinmalignantmelanomacells.



      PTM:N-glycosylated(Bysimilarity).&O-glycosylated;containsglycosaminoglycanchondroitinsulfatewhicharerequiredforproperlocalizationandfunctioninstressfiberformation(Bysimilarity).InvolvedininteractionwithMMP16andITGA4.&PhosphorylationbyPRKCAregulatesitssubcellularlocationandfunctionincellmotility(Bysimilarity).


      SIMILARITY:SwissProt:Q6UVK1##Contains15CSPG(NG2)repeats.&Contains2lamininG-likedomains.

      MISCELLANEOUS:ValuableMarkerforseveralincompletelydifferentiatedprecursorcells.
      MolecularWeight~270-300kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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