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Millipore/07-1238 | Anti-phospho-MARCKS (Ser152/156) Antibody/07-1238/100 µL
  • Millipore/07-1238 | Anti-phospho-MARCKS (Ser152/156) Antibody/07-1238/100 µL

Millipore/07-1238 | Anti-phospho-MARCKS (Ser152/156) Antibody/07-1238/100 µL

價格: ¥3324.00 市場價: 5540.00

貨號: 07-1238
品牌: Millipore
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    • Description
      CatalogueNumber07-1238
      ReplacesAB5879
      DescriptionAnti-phospho-MARCKS(Ser152/156)Antibody
      AlternateNames
      • Myristoylatedalanine-richC-kinasesubstrate
      • ProteinkinaseCsubstrate,80kDaprotein,lightchain
      BackgroundInformationMyristoylatedAlanine-RichC-KinaseSubstrate(MARCKS)isawidelyexpressedsubstrateofPKC.MARCKSisessentialinneuronaldevelopment,asmicedeficientinitdieshortlyafterbirth.Throughitsbasiceffectorregion,MARCKSassociateswithacidicmembranephospholipids,actinfilaments,andcalmodulin.Poly(ADP-ribose)alsobindsthebasiceffectorregion,blockingcalmodulinbinding,membraneassociation,andphosphorylationbyPKC,suggestingthatMARCKSmaybeaneffectoroftheDNA-damagesignaltransducedbypoly(ADP-ribose).
      ProductInformation
      FormatAffinityPurified
      Control
      • A431CellLysate
      PresentationPurifiedrabbitpolyclonalIgGin10mMHepes,pH7.5,with150mMNaCl,100μg/mLBSAand50%glycerol.
      StorageandShippingInformation
      StorageConditionsStableat-20°Cinundilutedforupto6monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.Donotstoreinaself-defrostingfreezer.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationThisAnti-phospho-MARCKS(Ser152/156)AntibodyisvalidatedforuseinWB,IH(P)forthedetectionofphospho-MARCKS(Ser152/156).
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry(Paraffin)
      ApplicationNotesImmunohistochemistry(paraffin):
      Representativeimagesfromapreviouslot.
      OptimalStainingofMARCKSWithCitratepH6.0EpitopeRetrieval:RatPlacenta

      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoaminoacidssurroundingthephosphoSer152/156ofratMARCKS.
      EpitopephosphoSer152/156
      HostRabbit
      SpecificityMARCKS,phosphoSer152/156.
      IsotypeIgG
      SpeciesReactivity
      • Rat
      • Human
      • Mouse
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisasubstrateforproteinkinaseC.Itislocalizedtotheplasmamembraneandisanactinfilamentcrosslinkingprotein.PhosphorylationbyproteinkinaseCorbindingtocalcium-calmodulininhibitsitsassociationwithactinandwiththeplasmamembrane,leADIngtoitspresenceinthecytoplasm.Theproteinisthoughttobeinvolvedincellmotility,phagocytosis,membranetraffickingandmitogenesis.
      GeneSymbol
      • MARCKS
      • MACS
      • 80K-L
      • PRKCSL
      • FLJ14368
      • phosphomyristin
      • MRACKS
      • PKCSL
      • FLJ90045
      Modifications
      • Phosphorylation
      PurificationMethodAffinityPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P29966#MARCKSisthemostprominentcellularsubstrateforproteinkinaseC.Thisproteinbindscalmodulin,actin,andsynapsin.MARCKSisafilamentous(F)actincross-linkingprotein.
      SIZE:332aminoacids;31555Da
      PTM:PhosphorylationbyPKCdisplacesMARCKSfromthemembrane.ItalsoinhibitstheF-actincross-linkingactivity.
      SIMILARITY:SwissProt:P29966##BelongstotheMARCKSfamily.
      MolecularWeight~80kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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