Description
MinimumEssentialMedium(MEM)isoneofthemostcommonlyusedofallcellculturemedia.MEMcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingHeLa,BHK-21,293,HEP-2,HT-1080,MCF-7,fibroblasts,andprimaryratastrocytes.Weofferavarietyof
Gibco®MEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisMEMismodifiedasfollows:With | Without |
•Hanks"salts | •SodiumBicarbonate |
•L-glutamine | |
•HEPES | |
Thecompleteformulationisavailable.
ProductIntendedUseFor
invitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.
ProductIntendedUseFor
invitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.
cGMPManufacturingGibco®MEMismanufacturedatacGMPcompliantfacilitylocatedinPaisley,Scotland,UK.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
MEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).ThisMEMformulationdoesnotrequireaCO
2environmenttomaintainphysiologicalpH.
PowderformsofGibco®cellculturemediumrequiresodiumbicarbonatesupplementation,pHadjustment,andfiltrationatthetimeofpreparation(seeprotocolfordetails).