GoldBio/CSF2 (GM-CSF), Human/20 μg/1120-03-20

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Human Colony Stimulating Factor 2 (granulocyte-macrophage)CSF2GM-CSFGMCSFMolgramostinMGI-1GM (Macrophage granulocyte inducer-1GM)

Colony Stimulating Factor 2 (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts, in response to cytokine or immune and inflammatory stimuli. CSF2 is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, CSF2 is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. It can induce human endothelial cells to migrate and proliferate and can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. CSF2 is species specific and human CSF2 has no biological effects on mouse cells.

Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture.

Sequence: APARSPSPSTQPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMMASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

MW: ~14.6 kDa (128 amino acid)

Purity: >96% by SDS-PAGEand HPLC analyses

Biological Activity: Fully biologically active when compared to standard. The ED₅₀ as determined by a assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0 × 10⁷ IU/mg.

Accession Number: P04141

HGNC ID: 2434

Source: Escherichia coli

Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using PBS that contains a 0.1% BSA to a concentration of0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C.Stock solutions should be aliquoted and stored at -80°C. Further dilutionsshould be made in appropriate buffered solutions containing BSA or serum.

Additional resources for this product may be found at:

OMIM

NOT FOR HUMAN USE!