Content:25columnsOverview
LSColumnsaredesignedforpositiveselectionofcells.Theyarealsosuitableforthedepletionofstronglymagneticallylabeledcells.
LSColumnscanbeusedwiththefollowingseparators:
- MidiMACS?Separator
- QuadroMACS?Separator
- VarioMACS?Separator,incombinationwithanLSColumnAdapter
- SuperMACS?IISeparator
- MultiMACSCell24SeparatorPlus,incombinationwiththeSingleColumnAdapter
Columncapacity
Forlymphoidcellsfromblood,bonemarrowortissue:
Upto108magneticallylabeledcellsfromupto2×109totalcells.
Forthecapacityofcellsfromothertissues,e.g.,neuraltissues,pleaserefertothedatasheetprovidedwiththeparticularMACSCellSeparationReagent.
ForusewiththeMultiMACSCell24SeparatorPlus:
Upto108magneticallylabeledcellsfromupto1×109totalcells.
Details
Backgroundinformation
ThematrixoftheLSColumnsiscomposedofferromagneticspheres,whicharecoveredwithacell-friendlycoatingallowingfastandgentleseparationofcells.WhenplacedinthemagneticfieldofaMACS
?Separator,thespheresamplifythemagneticfieldby10,000-fold,thusinducingahighgr
ADIentwithinthecolumn.ThisiscrucialforisolationofcellswhichareonlyminimallylabeledwithMACS
?MicroBeads,leavingenoughepitopesfreeforconcurrentantibodystaining.Thespacebetweenthespheresisseveraltimeslargerthanprimaryandmostculturedcells.Thisallowsthecellstofreelyflowthroughthecolumn.Magneticallylabeledcellsareheldins
USPensionwithinthecolumnanddonotactually“bind”thecolumnmatrix.Thissuspensionminimizesstressonthecellsandallowsforefficientsterilewashingbyavoidingcellaggregation.
LSColumnscanbeusedtoseparatemateriallessthan30μminsize.Thecolumnsareindividuallypackedinasterileway.