Capacity:for1×109totalhumancellsorfor2×109totalmousecellsOverview
CD11bMicroBeadsweredevelopedfortheisolationordepletionofhumanandmousecellsbasedontheirCD11bexpression.
Details
Backgroundinformation
Inhumans,CD11bisstronglyexpressedonmyeloidcells,andweaklyexpressedonNKcellsandsomeactivatedlymphocytes.
Inmice,theCD11bantigenisexpressedonmonocytes/macrophagesandtoalowerextentongranulocytes,NKcells,CD5+B1cells,andasubsetofdendriticcells.
Downstreamapplications
Inhumans,CD11bMicroBeadscanbeusedforpositiveselectionordepletionofCD11b+cellsfromPBMCs,bonemarrow,andotherlymphoidtissues.TheyaremostlyusedincombinationwithotherMicroBeadsfordepletionofunwantedcells.
Inmice,CD11bMicroBeadshavebeenusedtoisolateCD11b
+cellsfromcells
USPensionsofspleen
1,lymphnode
2,peritonealcavity
3,liver
4,muscletissue
2,orblood
5.TheywerealsousedincombinationwithotherMicroBeadsfortheenrichmentofmastcellsfromperitonealcellsuspensions.
3FortheisolationofhumanmonocytesCD14MicroBeadsorthePanMonocyteIsolationKitarerecommended.
Columns
Forpositiveselection:MS,LS,XS,orauto
MACS?Columns.Fordepletion:LD,CS,D,orautoMACSColumns.