EndotoxinRemovalBeadsrepresenttheidealtoolforfastandeasyremovalofendotoxinandDNAfrompurifiedproteinpreparations.
Preparationsofrecombinantproteinsareoftencontaminatedwithendotoxins(lipopolysaccharides,LPS)andDNAfromhostbacteria.BacterialendotoxinsandDNAshowstrong
BIOLOGicaleffectsinmanymammalsaswellasincellcultures.Ifpurifiedrecombinantproteinsareusedfor
invivoor
invitroassays,efficientremovalofendotoxinsandbacterialDNAisrequiredtoavoidnon-specificdetrimentaleffects.
ThecovalentlyboundpolycationicmoietyoftheEndotoxinRemovalBeadsshowsstrongbindingtobothendotoxinsandDNA.
EndotoxinorDNAcanberemovedby98%atinitialconcentrationsrangingfrom2000EU/mLto10,000EU/mLor2000to10,000ngofDNApermL,respectively.Endotoxinremovalisperformedusingthe
MACSiMAG?Separator.
Note:EndotoxinRemovalBeadsare
notsuitableforusewithMACS?ColumnsandMiniMACS?,MidiMACS?,VarioMACS?,SuperMACS?,autoMACS?,μMACS?,or
ThermoMACS?Separators.