Preparationsofrecombinantproteinsareoftencontaminatedwithendotoxins(lipopolysaccharides,LPS)andDNAfromhostbacteria.BacterialendotoxinsandDNAshowstrong
BIOLOGicaleffectsinmanymammalsaswellasincellcultures.Ifpurifiedrecombinantproteinsareusedfor
invivoor
invitroassays,efficientremovalofendotoxinsandbacterialDNAisrequiredtoavoidnon-specificdetrimentaleffects.