ThemouseAnti-Gr-1antibodyreactswithLy-6G,a21-25kDa,GPI-anchoredcellsurfaceprotein,previouslydefinedasthegranulocyte-differentiationantigen-1(Gr-1).1Theantibodyhasbeenshowntocross-reactweaklywithLy-6C-transfectedEL-4Jcells.2Cross-reactivityofAnti-Gr-1withLy-6CwasnotdetectedonhematopoieticcellsthatexpressLy-6CandarenegativeforLy-6G.3
Gr-1isexpressedonmaturegranulocytesinbonemarrowandperipheraltissues.TheAnti-Gr-1antibodyalsostainsmonocytestransientlyduringtheirdifferentiationinbonemarrowandatlowlevelsplasmacytoiddendriticcellsinlymphoidtissues.
Whenusingformaldehyde-fixedcellsthatexpressGr-1athighlevels,forexample,granulocytes,thedilutionis1:50forupto10?cells/100?μLofbufferforlabelingofcellsandanalyzingbyflowcytometry.