Exosomeareincubatedwiththeantibody-coatedMACSPlexExosomeCaptureBeads.Subsequentlyorinparallel,exosomesboundtotheMACSPlexExosomeCaptureBeadsarelabeledwiththeMACSPlexExosomeDetectionReagents.TheMACSPlexExosomeDetectionReagentscanalsobecombinedtocreateacocktailcomprisingofMACSPlexExosomeDetectionReagentforCD9,CD63,andCD81.Consequently,sandwichcomplexesareformedbetweentheMACSPlexExosomeCaptureBead,exosome,andthedetectionreagent(seeGallery,Figure1).ThesecomplexescanbeanalyzedbasedonthefluorescencecharacteristicsofboththeMACSPlexExosomeCaptureBeadandthedetectionreagent.Positivesignalsindicatethepresenceoftherespectivesurfaceepitopewithintheexosomepopulation(seeGallery,Figure2).Itisalsoposs
IBLetocomparedifferentexosomesamplesusingtheMACSPlexExosomeKitallowingsemi-quantitativeanalysisofdifferentialsurfaceepitopes.
TheMACSPlexExosomeKitallowsforqualitativeandsemiquantitativeanalysisof37exosomalsurfaceepitopes.TheMACSPlexExosomeKithasbeendevelopedforthesimultaneousflowcytometricdetectionof37surfaceepitopesthatareknowntobepresentondifferentexosomesplustwoisotypecontrolbeads.