TheFFPETissueDissociationKithasbeenoptimizedforthegentle,rapid,andeffectivegenerationofsingle-cells
USPensionsfromhumanformalin-fixedparaffin-embedded(FFPE)carcinomasections.Thus,ahighyieldofsingle?cellscanbeobtained,whilepreservingtheimportantcellepitopescytokeratinforcellanalysisofcarcinomacellsandvimentinfornon-carcinomacells.
Dissociatedcellscanbesubsequentlyseparatedbyflowsortingoranalyzedbyflowcytometryinvitroforphenotypedistributions,andothergeneticorproteomicstudies.