Source | ExpressedinE.coli |
SpecificActivity | ForSpecificActivitydata,refertotheCertificateofAnalysisforindividuallotsofthisenzyme. |
EntrezGeneNumber | |
EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheMAPkinasefamily.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actasanintegrationpointformultiplebiochemicalsignals,andareinvolvedinawidevarietyofcellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment.Theactivationofthiskinaserequiresitsphosphorylationbyupstreamkinases.Uponactivation,thiskinasetranslocatestothenucleusofthestimulatedcells,whereitphosphorylatesnucleartargets.Twoalternativelysplicedtranscriptvariantsencodingthesameprotein,butdifferingintheUTRs,havebeenreportedforthisgene. |
GeneSymbol | - MAPK1
- p40
- PRKM2
- MAPK2
- PRKM1
- p42-MAPK
- p38
- p41
- ERK
- ERK-2
- ERT1
- P42MAPK
- p41mapk
- ERK2
|
ProteinTarget | MAPK1 |
PurificationMethod | Purifiedbyglutathione-agarose.ActivatedwithMEK1andrepurifiedonNi2+/NTAagarose |
TargetSub-Family | CMGC |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P28482#Involvedinboththeinitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactorssuchasELK1.PhosphorylatesEIF4EBP1;requiredforinitiationoftranslation.Phosphorylatesmicrotubule-associatedprotein2(MAP2).PhosphorylatesSPZ1(Bysimilarity).Phosphorylatesheatshockfactorprotein4(HSF4). COFACTOR:Magnesium(Bysimilarity). SIZE:360aminoacids;41390Da SUBUNIT:InteractswithMORG1(Bysimilarity).BindstoHIV-1NefthroughitsSH3domain.Thisinteractioninhibitsitstyrosine-kinaseactivity.InteractswithHSF4. DOMAIN:SwissProt:P28482TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases. PTM:DuallyphosphorylatedonThr-185andTyr-187,whichactivatestheenzyme. SIMILARITY:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.&Contains1proteinkinasedomain. |
MolecularWeight | 69.9kDa |