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Millipore/17-10101 | ChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set/17-10101/25 assays25 ass
  • Millipore/17-10101 | ChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set/17-10101/25 assays25 ass

Millipore/17-10101 | ChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set/17-10101/25 assays25 ass

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貨號: 17-10101
品牌: Millipore
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    • Description
      CatalogueNumber17-10101
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+Acetyl-HistoneH4(Lys16)-ChIPValidatedAntibodyandPrimerSet
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+Acetyl-HistoneH4(Lys16)setincludestheAcetyl-HistoneH4(Lys16)antibody,anegativecontrolnormalrabbitIgG,andqPCRprimerswhichamplifya178bpregionofhumanRPL10promoter.TheAcetyl-HistoneH4(Lys16)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofAcetyl-HistoneH4(Lys16)-associatedchromatin.
      AlternateNames
      • H4K16Ac
      • HistoneH4(acetylK16)
      • H4histonefamily,memberA
      • histone1,H4a
      • histonecluster1,H4a
      BackgroundInformationHistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.AcetylationofhistoneH4occursatseveraldifferentlysinepositionsinthehistonetailandisperformedbyafamilyofenzymesknownasHistoneAcetylTransferases(HATs).
      ProductInformation
      FormatPurified
      Control
      • IncludesnegativecontrolnormalrabbitIgGandprimersspecificforhumanRPL10promoter.
      PresentationAnti-Acetyl-HistoneH4(Lys16)(rabbitpolyclonal).Onevialcontaining50μLofaffinitypurifiedrabbitpolyclonalseruminbuffercontaining0.1MTris-Glycine(pH7.4)150mMNaClwith0.05%sodiumazide.Storeat-20°C.

      NormalRabbitIgG.Onevialcontaining125μgofrabbitIgGin125μLofstoragebuffercontaining0.05%sodiumazide.Storeat-20°C.

      ChIPPrimers,RPL10promoter.Onevialcontaining75μLof5μMofeachprimerspecificforthepromoterregionofhumanRPL10.Storeat-20°C.
      FOR:ACCCGTCTTCGACAGGACT
      REV:GGAACGGAAGACGAGAACAG
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationThisChIPAb+Acetyl-HistoneH4(Lys16)-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • Immunoprecipitation
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      Representativelotdata.
      SonicatedchromatinpreparedfromHeLacells(1X10E6cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither2μgofNormalrabbitIgGor2μLofAnti-acetyl-HistoneH4(Lys16)andtheMagnaChIP?AKit(Cat.#17-610).
      Successfulimmunoprecipitationofacetyl-HistoneH4(Lys16)associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,RPL10Promoterasapositivelocus,andβ-Globinprimersasanegativelocus.(Pleaseseefigures).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
      PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      Representativelotdata.
      RecombinantHistoneH4(Cat#14-697)(lane1)andHeLaacidextract(lane2)wasresolvedbyelectrophoresis,transferredtoPVDFandprobedwithanti-AcetylHistoneH4(Lys16)(0.1μg/mL).
      Proteinswerevisualizedusingadonkeyanti-rabbitsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem.
      ArrowindicatesAcetylHistoneH4(Lys16)(~10kDa)(Pleaseseefigures).

      Multiplexing(Luminex?):
      Representativelotdata.
      ThisantibodyspecificallyrecognizeshistoneH4acetylatedonLys16byLuminexassay(Pleaseseefigures).

      PeptideInhibitionAssay:
      Representativelotdata.
      ThisantibodypeptideblockedonHeLacellextracts(Pleaseseefigures).
      BiologicalInformation
      ImmunogenKLH-conjugatedHistoneH4correspondingtotheN-terminusregionatandaroundacetylatedLys16.
      EpitopeAcetylatedH4Lys16
      HostRabbit
      SpecificityThisantibodydetectshistoneH4acetylatedatLys16.
      SpeciesReactivity
      • Human
      • Vertebrates
      SpeciesReactivityNoteHuman.Broadspeciescross-reactivityisexpectedbasedonsequencehomology.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6.
      GeneSymbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      PurificationMethodAffinityPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:103aminoacids;11367Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.
      SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family.
      MolecularWeight~10kDaobserved
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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