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Millipore/17-630 | ChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set/17-630/25 assays25 assays per set.
  • Millipore/17-630 | ChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set/17-630/25 assays25 assays per set.

Millipore/17-630 | ChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set/17-630/25 assays25 assays per set.

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貨號: 17-630
品牌: Millipore
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    • Description
      CatalogueNumber17-630
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+Acetyl-HistoneH4-ChIPValidatedAntibodyandPrimerSet
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+Acetyl-HistoneH4setincludespurifiedrabbitpolyclonalantiserumandthenormalrabbitIgGantiserum,whichcanbeusedtodemonstratethattheacetyl-histoneH4antibodyiscapableofprecipitatingacetyl-histoneH4associatedchromatin.TheqPCRprimersincludedflankthehumanGAPDHpromoterandproducea166basepairPCRproduct.
      ProductInformation
      FormatSerum
      Control
      • IncludednegativecontrolrabbitIgGantiserumandcontrolprimersspecificforhumanGAPDHpromoter.
      PresentationAnti-Acetyl-HistoneH4(rabbitpolyclonalIgG).Onevialcontaining100μLofantiserumcontaining0.05%sodiumazide.Storeat-20°C.

      NormalRabbitSerum.Onevialcontaining100uLantiserumcontaining0.05%sodiumazide.Storeat-20°C.

      ControlPrimers.Onevialcontaining75μLof5μMofeachprimerspecificforforhumanGAPDH.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.
      Applications
      ApplicationThisChIPAb+Acetyl-HistoneH4-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • WesternBlotting
      • Multiplexing
      • CellFunctionAssay
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesWesternBlotAnalysis:
      AcidextractedproteinsfromnormalHeLacells(Lane1)andHeLacellstreatedwith5mMsodiumbutyratefor24hours(Lane2)wereresolvedbyelectrophoresis,transferredtonitrocelluloseandprobedwithanti-acetylHistoneH4(1:2000).
      Proteinswerevisualizedusingagoat-antirabbitsecondaryantibodyconjugatedtoHRPandachemi-luminescencedetectionsystem.(Pleaseseefigures).
      BiologicalInformation
      ImmunogenTheacetyl-histoneH4antiserumismadeagainstapeptidecorrespondingtoaminoacids2-19ofTetrahymenahistoneH4.
      HostRabbit
      SpecificityRecognizesacetylatedhistoneH4ofapproximately10kDa.Cross-reactswithacetylatedhistoneH2BfromTetrahymenaandweaklycrossreactswithacetylatedhistoneH2BfromHeLacells.
      Maycross-reactwithotheracetylatedproteins.
      SpeciesReactivity
      • Human
      • Eukaryote
      SpeciesReactivityNoteProventoreactwithhumansamples.Predictedbroadcross-reactivityamongeukaryotesbaseduponsequencesimilarity.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      UniProtNumber
      UniProtSummaryFunction:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      Subunitstructure:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      Subcellularlocation:Nucleus.
      Post-translationalmodification:AcetylationatLys-6(H4K5ac),Lys-9(H4K8ac),Lys-13(H4K12ac)andLys-17(H4K16ac)occursincodingregionsofthegenomebutnotinheterochromatin.
      CitrullinationatArg-4(H4R3ci)byPADI4impairsmethylation.
      MonomethylationatArg-4(H4R3me1)byPRMT1favorsacetylationatLys-9(H4K8ac)andLys-13(H4K12ac).DemethylationisperformedbyJMJD6.
      SymmetricdimethylationonArg-4(H4R3me2a)bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage.
      Monomethylated,dimethylatedortrimethylatedatLys-21(H4K20me1,H4K20me2,H4K20me3).MonomethylationisperformedbySET8.TrimethylationisperformedbySUV420H1andSUV420H2andinducesgenesilencing.
      UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirradiation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.MonoubiquitinatedatLys-92ofhistoneH4(H4K91ub1)inresponsetoDNAdamage.TheexactroleofH4K91ub1inDNAdamageresponseisstillunclearbutitmayfunctionasalicensingsignalforadditionalhistoneH4post-translationalmodificationssuchasH4Lys-21methylation(H4K20me).
      Sumoylated,whichisassociatedwithtranscriptionalrepression.
      Sequencesimilarities:BelongstothehistoneH4family.

      MolecularWeight10kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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