Coumarin343isablueemittingfluorophorewithanemissionmaximumaround480nm.ThisdyeformsaFRETpairwithfluorescein,andcanharvestbluelightenergyforthesubsequenttransfertootherfluorophores.
Theazidederivativecanbeconjugatedwithalkynesinacopper-catalyzedandcopper-freeClickchemistryreactions.Themoleculecontainsalongaminohexanoyllinkerthatprovidesseparationbetweenthedye,andtheazidefunction.
Generalproperties
Appearance: | yellowsolid |
MassspecM+increment: | 480.3 |
Molecularweight: | 480.56 |
Molecularformula: | C25H32N6O4 |
IUPACname: | 5-{[6-(3-Azidopropylamino)-6-oxohexylamino]carbonyl}-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-4-one |
Solubility: | goodinDMF,DMSO |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
Excitationmaximum,nm: | 437 |
ε,L⋅mol−1⋅cm−1: | 44000 |
Emissionmaximum,nm: | 477 |
Fluorescencequantumyield: | 0.63 |
CF260: | 0.29 |
CF280: | 0.24 |