CatalogueNumber | 2760 |
BrandFamily | Chemicon®
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TradeName | |
Description | BrdUIHCKit |
Overview | TestPrinciple
CHEMICON?"sBrdUImmunohistochemistryKitisanon-isotopicimmuno-histochemistrystainingkitforthedetectionandlocalizationofBrdUincorporatedintonewlysynthesizedDNAofproliferatingcells.VisualstainingofthecellsisachievedbyusingabiotinylatedmonoclonalantibodytoBrdUfollowedbystreptavidin-HRPconjugateandDABsubstrate. |
BackgroundInformation | Evaluationofcellcycleprogressionisessentialforinvestigationsinmanyscientificfields.Measurementof[3H]thymidineincorporationascellsenterSphasehasbeenatrADItionalmethodfordetectionofcellproliferation.Subsequentquantificationof[3H]thymidineisperformedbyscintillationcountingofautoradiography.Thistechnologyisslow,laborintensiveandhasseverallimitationsincludingthehandlinganddisposalofradioisotopesandthenecessityofexpensiveequipment.
Awellestablishedalternativeto[3H]thymidineuptakehasbeendemonstratedbynumerousresearchers.Inthesemethodsbromodeoxyuridine(BrdU),athymidineanalog,replaces[3H]thymidine.BrdUisincorporatedintonewlysynthesizedDNAstrandsofactivelyproliferatingcells.FollowingpartialdenaturationofdoublestrandedDNA,BrdUisdetectedimmunochemicallyallowingtheassessmentofthepopulationofcellswhicharesynthesizingDNA. |
MaterialsRequiredbutNotDelivered | 1.QuenchingSolution:Hydrogenperoxide(30%)solutionforquenchingendogenousperoxidaseactivity.
2.Phosphatebufferedsaline(PBS)solution.
3.Distilledwater.
4.Ethylalcohol
5.Xylene
6.Coverslips
7.Bromodeoxyuridine(BrdU)
8.Methanol |