FreeCyanine3carboxylicacid(Cy3®carboxylicacidanalog),non-activateddye.Non-sulfonatedreagent,withgoodsolubilityinorganicsolvents,andlimitedaquoussolubility.Thedyecanbeusedasanon-reactivefluorophore,forcontrolexperiments,andforcalibration.
Forcouplingwithamines,andlabeling,considerusingCyanine3NHSester,orwater-solublesulfo-Cyanine3NHSester.
Generalproperties
Appearance: | redpowder |
Molecularweight: | 493.08 |
CASnumber: | 1144107-76-5,1361402-15-4,1251915-29-3[I-] |
Molecularformula: | C30H37ClN2O2 |
Solubility: | solubleinorganicsolvents(DMF,DMSO,dichloromethane),insolubleinwater |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
Excitationmaximum,nm: | 555 |
ε,L⋅mol−1⋅cm−1: | 150000 |
Emissionmaximum,nm: | 570 |
Fluorescencequantumyield: | 0.31 |
CF260: | 0.04 |
CF280: | 0.09 |