A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography.
An analog without sulfo groups, Cyanine5 maleimide, is also available.
Download in
ChemDraw format
General properties
Appearance: | dark blue powder |
Molecular weight: | 803.00 |
Molecular formula: | C38H43KN4O9S2 |
Solubility: | soluble in water, DMSO, DMF |
Quality control: | NMR 1H, HPLC-MS (95+%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
Excitation maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 271000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.28 |
CF260: | 0.04 |
CF280: | 0.04 |