Discovery BIO Wide Pore (300 ?) C18,
Discovery Wide Pore C18 HPLC Columns for proteins,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations,
HPLC Columns, by Phase,
Reversed Phase HPLC Columns for Biomolecules,
Wide Pore C18 columns for large molecules
More...
feature
endcapped
extent of labeling
9.2% Carbon loading
application(s)
HPLC: suitable
L × I.D.
15 cm × 2.1 mm
surface coverage
3.6 μmol/m2
matrix
silica particle platform
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
300 ?
surface area
100 m2/g
suitability
suitable for L1 per USP
mode of chromatography
Reversed phase
Description:
General description
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
Recommended products
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Discovery is a registered trademark of Sigma-Aldrich Co. LLC