Watersoluble,amino-reactivesulfo-Cyanine3NHSester.Efficientlylabelsproteinsandpeptidesinpurelyaqueoussolution,withoutneedfororganicco-solvent.Idealforproteinswithlowsolubility,andproteinspronetodenaturation.
Thisissulfonated,hydrophilicandwater-solubledye.Non-sulfonatedCyanine3NHSesterisalsoavailable.
ThisproductisananalogofCy3®NHSester.Sulfo-Cyanine3NHSesterreplacesCy3®,AlexaFluor546,andDyLight549forallapplications.
Generalproperties
Appearance: | darkredcrystals |
Molecularweight: | 735.80 |
Molecularformula: | C34H38N3NaO10S2 |
Solubility: | veryhighinwater,goodinpolarorganicsolvents(DMF,DMSO) |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
Excitationmaximum,nm: | 548 |
ε,L⋅mol−1⋅cm−1: | 162000 |
Emissionmaximum,nm: | 563 |
Fluorescencequantumyield: | 0.1 |
CF260: | 0.03 |
CF280: | 0.06 |