BioMag® anti-Human CD IgG-coated particles are produced by covalently attaching murine monoclonal antibodies to BioMag® particles. The antibodies were prepared against human leukocyte differentiation antigens. Each product is supplied in phosphate buffered saline with EDTA and sodium azide added as stabilizers, and should be washed in sterile media prior to use to remove the preservative.
The CD14 antigen is found on cells of myelomonocytic lineage and is stronly expressed on monocytes and macrophages. CD14 is also found on Langerhans cells, follicular dendritic cells and histiocytes and is weakly expressed on B lymphocytes and neutrophils, but not expressed on T lymphocytes, NK cells, red blood cells and platelets.