Synonym:
2-Amino-2-(hydroxymethyl)-1,3-propanediol,
THAM,
Tris base,
Tris(hydroxymethyl)aminomethane,
Trometamol
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CAS Number 77-86-1
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Linear Formula NH2C(CH2OH)3
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Molecular Weight 121.14
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Beilstein Registry Number 741883
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EC Number 201-064-4
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MDL number MFCD00004679
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PubChem Substance ID 57653537
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Popular Documents: Datasheet (PDF)
Properties:
Related Categories |
Analytical Reagents,
Analytical/Chromatography,
Biochemicals and Reagents,
Biological Buffers,
Buffers A to Z,
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description
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aminopeptidase substrate
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InChI Key
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LENZDBCJOHFCAS-UHFFFAOYSA-N
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grade
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puriss. p.a.
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assay
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≥99.7% (T)
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form
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crystalline
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ign. residue
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≤0.01% (as SO4)
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color
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white
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pH
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10.5-11.5 (25 °C, 10% in H2O)
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useful pH range
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7 - 9
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pKa (25 °C)
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8.1
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bp
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219-220 °C/10 mmHg(lit.)
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mp
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167-172 °C(lit.)
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168-172 °C
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solubility
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water: soluble (678 g/l at 20 °C)
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anion traces
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chloride (Cl-): ≤5 mg/kg
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sulfate (SO42-): ≤5 mg/kg
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cation traces
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Ca: ≤10 mg/kg
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Cd: ≤5 mg/kg
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Co: ≤5 mg/kg
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Cr: ≤5 mg/kg
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Cu: ≤5 mg/kg
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Fe: ≤5 mg/kg
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K: ≤50 mg/kg
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Mg: ≤5 mg/kg
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Mn: ≤5 mg/kg
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Na: ≤50 mg/kg
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Ni: ≤5 mg/kg
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Pb: ≤5 mg/kg
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Zn: ≤5 mg/kg
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λ
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10 % in H2O
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UV absorption
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λ: 230 nm Amax: ≤0.6
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λ: 260 nm Amax: ≤0.05
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λ: 280 nm Amax: ≤0.04
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λ: 430 nm Amax: ≤0.01
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Description:
Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Safety:
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
RIDADR
NONH for all modes of transport