Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | ADD1(Phospho-Ser726)Antibody |
Synonyms | Alpha-adducin;Erythrocyteadducinsubunitalpha;ADD1;ADDA |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanADD1aroundthephosphorylationsiteofSer726. |
ImmunogenRange | 688-737 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:40000 |
Specificity | ADD1(Phospho-Ser726)AntibodydetectsendogenouslevelsofADD1onlywhenphosphorylatedatSer726. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | ADD1 |
DatabaseLinks | GeneID: 118/119 Swiss-Prot#: P35611/P35612 OMIM#: 102680/102681 Unigene#: Hs.183706/Hs.701490/Hs.188528 |
TargetModification | Phospho |
ModificationSites | H:S726/S713M:S724/S712R:S724/S712 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHeLacells,usingADD1(Phospho-Ser726)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingADD1(Phospho-Ser726)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromHeLacellstreatedwithForskolin40nM30",usingADD1(Phospho-Ser726)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |