Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | CREB(Phospho-Ser142)Antibody |
Synonyms | cAMPresponsiveelementbindingprotein1;cAMP-responseelementbindingprotein;CREB-1;CREB1 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCREBaroundthephosphorylationsiteofSer142. |
ImmunogenRange | 111-160 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:40000 |
Specificity | CREB(Phospho-Ser142)AntibodydetectsendogenouslevelsofCREBonlywhenphosphorylatedatSer142. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | CREB1 |
DatabaseLinks | GeneID: 1385 Swiss-Prot#: P16220 OMIM#: 123810/612160 Unigene#: Hs.516646/Hs.584750 |
TargetModification | Phospho |
ModificationSites | H:S142M:S142R:S142 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHeLacells,usingCREB(Phospho-Ser142)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanlungcarcinoma,usingCREB(Phospho-Ser142)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromHeLacellstreatedwithPMA125ng/ml30",usingCREB(Phospho-Ser142)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |