Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | DARPP-32(Phospho-Thr75)Antibody |
Synonyms | Dopamine-andcAMP-regulatedneuronalphosphoprotein;IPPD;NeuronalphosphoproteinDARPP-32;PPP1R1B |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanDARPP-32aroundthephosphorylationsiteofThr75. |
ImmunogenRange | 41-90 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:40000 |
Specificity | DARPP-32(Phospho-Thr75)AntibodydetectsendogenouslevelsofDARPP-32onlywhenphosphorylatedatThr75. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | PPP1R1B |
DatabaseLinks | GeneID: 84152 Swiss-Prot#: Q9UD71 OMIM#: 604399 Unigene#: Hs.286192 |
TargetModification | Phospho |
ModificationSites | H:T75M:T75R:T75 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingDARPP-32(Phospho-Thr75)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromCOS7cellstreatedwithForskolin40nM30",usingDARPP-32(Phospho-Thr75)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |