IkappaB-beta (Phospho-Ser23) Antibody detects endogenous levels of IkappaB-beta only when phosphorylated at Ser23.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NFKBIB
Database Links
Gene ID: 4793 Swiss-Prot #: Q15653
OMIM #: 604495 Unigene #: Hs.9731
Target Modification
Phospho
Modification Sites
H:S23 M:S23 R:S23
Application Images
Image 1
Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 5', using IkappaB-beta (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide.