Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | Caspase9(Phospho-Thr125)Antibody |
Synonyms | APAF-3;ApoptoticproteaseMch-6;Apoptoticproteaseactivatingfactor3;CASP-9;Caspase-9;Caspase-9precursor;Caspase9;ICE-LAP6;ICE-likeapoptoticprotease6;MCH6;RNCASP9 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCaspase9aroundthephosphorylationsiteofThr125. |
ImmunogenRange | 91-140 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:5000 |
Specificity | Caspase9(Phospho-Thr125)AntibodydetectsendogenouslevelsofCaspase9onlywhenphosphorylatedatThr125. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | CASP9 |
DatabaseLinks | GeneID: 842 Swiss-Prot#: P55211 OMIM#: 602234 Unigene#: Hs.329502 |
TargetModification | Phospho |
ModificationSites | H:T125M:T163R:T163 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanlungcarcinoma,usingCaspase9(Phospho-Thr125)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromHeLacellstreatedwithTNF20ng/ml5"+calyculinA50ng/ml5",usingCaspase9(Phospho-Thr125)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |