The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser126.
Immunogen Range
91-140
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
Cyclin B1 (Phospho-Ser126) Antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Ser126.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CCNB1
Database Links
Gene ID: 891 Swiss-Prot #: P14635
OMIM #: 123836 Unigene #: Hs.23960
Target Modification
Phospho
Modification Sites
H:S126 M:S123 R:S116
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Cyclin B1 (Phospho-Ser126) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 15', using Cyclin B1 (Phospho-Ser126) Antibody. The lane on the right is blocked with the phospho peptide.