The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
STAT5B (Phospho-Ser731) Antibody
Synonyms
Signal transducer and activator of transcription 5B
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT5B around the phosphorylation site of Ser731.
Immunogen Range
697-746
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
STAT5B (Phospho-Ser731) Antibody detects endogenous levels of STAT5B only when phosphorylated at Ser731.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
STAT5B
Database Links
Gene ID: 6777 Swiss-Prot #: P51692
OMIM #: 245590/604260 Unigene #: Hs.714309
Target Modification
Phospho
Modification Sites
H:S731 M:S730 R:S730
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT5B (Phospho-Ser731) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using STAT5B (Phospho-Ser731) Antibody. The lane on the right is blocked with the phospho peptide.