The antiserum was produced against synthesized peptide derived from human SYK around the phosphorylation site of Tyr525.
Immunogen Range
491-540
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
SYK (Phospho-Tyr525) Antibody detects endogenous levels of SYK only when phosphorylated at Tyr525.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SYK
Database Links
Gene ID: 6850 Swiss-Prot #: P43405
OMIM #: 600085 Unigene #: Hs.371720
Target Modification
Phospho
Modification Sites
H:Y525 M:Y519 R:Y519
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SYK (Phospho-Tyr525) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from A549 cells, using SYK (Phospho-Tyr525) Antibody. The lane on the right is blocked with the phospho peptide.