The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
WNK1 (Phospho-Thr58) Antibody
Synonyms
WNK lysine deficient protein kinase 1; KDP; PRKWNK1; PROTEIN KINASE; LYSINE-DEFICIENT 1
Immunogen
The antiserum was produced against synthesized peptide derived from human WNK1 around the phosphorylation site of Thr58.
Immunogen Range
24-73
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
WNK1 (Phospho-Thr58) Antibody detects endogenous levels of WNK1 only when phosphorylated at Thr58.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WNK1 (Phospho-Thr58) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using WNK1 (Phospho-Thr58) Antibody. The lane on the right is blocked with the phospho peptide.