EasyProbes? Cell Viability Imaging Kit (Blue/Red) is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of EasyProbes? Hoechst 33342 Live Cell Stain reagent and EasyProbes??Propidium Iodide Dead?Cell Stain reagent to 1 mL of cell growth media, then determine viability by counting total vs dead cells. EasyProbes? Hoechst 33342 reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. EasyProbes??PI reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard TRITC/RFP (orange) filter set. This kit is appropriate for fluorescence microscopy and flow cytometry.