The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser124.
Immunogen Range
90-139
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
Akt (Phospho-Ser124) Antibody detects endogenous levels of Akt only when phosphorylated at Ser124.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AKT1
Database Links
Gene ID: 207 Swiss-Prot #: P31749
OMIM #: 164730 Unigene #: Hs.525622
Target Modification
Phospho
Modification Sites
H:S124 M:S124 R:S124
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser124) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from mouse brain, using Akt (Phospho-Ser124) Antibody. The lane on the right is blocked with the phospho peptide.