The antiserum was produced against synthesized peptide derived from human ATF1 around the phosphorylation site of Ser63.
Immunogen Range
31-80
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
ATF1 (Phospho-Ser63) Antibody detects endogenous levels of ATF1 only when phosphorylated at Ser63.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ATF1
Database Links
Gene ID: 466 Swiss-Prot #: P18846
OMIM #: 123803/612160 Unigene #: Hs.648565
Target Modification
Phospho
Modification Sites
H:S63 M:S63
Application Images
Image 1
Western blot analysis of lysates from HT29 cells treated with Insulin 0.01U/ML 15', using ATF1 (Phospho-Ser63) Antibody. The lane on the right is blocked with the phospho peptide.