The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta around the phosphorylation site of Ser180/181.
Immunogen Range
151-200
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
IKK-alpha/beta (Phospho-Ser180/181) Antibody detects endogenous levels of IKK-alpha/beta only when phosphorylated at Ser180/181.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The lane on the right is blocked with the phospho peptide.