The antiserum was produced against synthesized peptide derived from human Akt1 S1 around the phosphorylation site of Thr246.
Immunogen Range
207-256
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Akt1 S1 (Phospho-Thr246) Antibody detects endogenous levels of Akt1 S1 only when phosphorylated at Thr246.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AKT1S1
Database Links
Gene ID: 84335 Swiss-Prot #: Q96B36
OMIM #: 610221 Unigene #: Hs.515542
Target Modification
Phospho
Modification Sites
H:T246 M:T247 R:T262
Application Images
Image 1
Western blot analysis of lysates from HepG2 cells treated with PDGF 50ng/ml 30', using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is blocked with the phospho peptide.