The antiserum was produced against synthesized peptide derived from human Arrestin 1 around the phosphorylation site of Ser412.
Immunogen Range
369-418
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
Arrestin 1 (Phospho-Ser412) Antibody detects endogenous levels of Arrestin 1 only when phosphorylated at Ser412.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ARRB1
Database Links
Gene ID: 408 Swiss-Prot #: P49407
OMIM #: 107940 Unigene #: Hs.503284/Hs.625320
Target Modification
Phospho
Modification Sites
H:S412
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Arrestin 1 (Phospho-Ser412) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with Etoposide 25uM 60', using Arrestin 1 (Phospho-Ser412) Antibody. The lane on the right is blocked with the phospho peptide.