The antiserum was produced against synthesized peptide derived from human Aurora Kinase around the phosphorylation site of Thr288.
Immunogen Range
256-305
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
Aurora Kinase (Phospho-Thr288) Antibody detects endogenous levels of Aurora Kinase only when phosphorylated at Thr288.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AURKA
Database Links
Gene ID: 6790 Swiss-Prot #: O14965
OMIM #: 603072 Unigene #: Hs.250822
Target Modification
Phospho
Modification Sites
H:T288 M:T279 R:T281
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Aurora Kinase (Phospho-Thr288) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with serum 20% 15', using Aurora Kinase (Phospho-Thr288) Antibody. The lane on the right is blocked with the phospho peptide.