Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | Calnexin(Phospho-Ser583)Antibody |
Synonyms | CALX;CANX;IP90;MajorhistocompatibilitycomplexclassI |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCalnexinaroundthephosphorylationsiteofSer583. |
ImmunogenRange | 543-592 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:5000 |
Specificity | Calnexin(Phospho-Ser583)AntibodydetectsendogenouslevelsofCalnexinonlywhenphosphorylatedatSer583. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | CANX |
DatabaseLinks | GeneID: 821 Swiss-Prot#: P27824 OMIM#: 114217 Unigene#: Hs.699155 |
TargetModification | Phospho |
ModificationSites | H:S583M:S582R:S582 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHeLacells,usingCalnexin(Phospho-Ser583)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingCalnexin(Phospho-Ser583)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromHeLacellstreatedwithEGF200ng/ml30",usingCalnexin(Phospho-Ser583)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |