IRF-3 (Phospho-Ser385) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser385.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
IRF3
Database Links
Gene ID: 3661 Swiss-Prot #: Q14653
OMIM #: 603734 Unigene #: Hs.75254
Target Modification
Phospho
Modification Sites
H:S385
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.