MDM2 (Phospho-Ser166) Antibody detects endogenous levels of MDM2 only when phosphorylated at Ser166.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MDM2
Database Links
Gene ID: 4193 Swiss-Prot #: Q00987
OMIM #: 164785 Unigene #: Hs.567303
Target Modification
Phospho
Modification Sites
H:S166 M:S163
Application Images
Image 1
Immunofluorescence analysis of A549 cells, using MDM2 (Phospho-Ser166) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MDM2 (Phospho-Ser166) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from COS7 cells, using MDM2 (Phospho-Ser166) Antibody. The lane on the right is blocked with the phospho peptide.