The antiserum was produced against synthesized peptide derived from human NMDAR2B around the phosphorylation site of Tyr1474.
Immunogen Range
1435-1484
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
NMDAR2B (Phospho-Tyr1474) Antibody detects endogenous levels of NMDAR2B only when phosphorylated at Tyr1474.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
GRIN2B
Database Links
Gene ID: 2904 Swiss-Prot #: Q13224
OMIM #: 138252 Unigene #: Hs.654430
Target Modification
Phospho
Modification Sites
H:Y1474 M:Y1472 R:Y1472
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right is blocked with the phospho peptide.