p53 (Phospho-Ser392) Antibody detects endogenous levels of p53 only when phosphorylated at Ser392.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of HeLa cells, using p53 (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using p53 (Phospho-Ser392) Antibody. The lane on the right is blocked with the phospho peptide.