The antiserum was produced against synthesized peptide derived from human TIE2 around the phosphorylation site of Tyr1108.
Immunogen Range
1074-1123
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
Specificity
TIE2 (Phospho-Tyr1108) Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr1108.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
TEK
Database Links
Gene ID: 7010 Swiss-Prot #: Q02763
OMIM #: 600195/600221 Unigene #: Hs.89640
Target Modification
Phospho
Modification Sites
H:Y1108 M:Y1106
Application Images
Image 1
Immunofluorescence analysis of HepG2 cells, using TIE2 (Phospho-Tyr1108) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells, using TIE2 (Phospho-Tyr1108) Antibody. The lane on the right is blocked with the phospho peptide.