The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser129.
Immunogen Range
95-144
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
Akt (Phospho-Ser129) Antibody detects endogenous levels of Akt only when phosphorylated at Ser129.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AKT1
Database Links
Gene ID: 207 Swiss-Prot #: P31749
OMIM #: 164730 Unigene #: Hs.525622
Target Modification
Phospho
Modification Sites
H:S129 M:S129 R:S129
Application Images
Image 1
Western blot analysis of lysates from A549 cells treated with PMA 125ng/ml 30', using Akt (Phospho-Ser129) Antibody. The lane on the right is blocked with the phospho peptide.